KMID : 0614620080520060359
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Korean Journal of Gastroenterology 2008 Volume.52 No. 6 p.359 ~ p.367
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Immunohistochemical Analysis of Nuclear Factor, p38, and Cyclin D1 Proteins in Premalignant Lesions and Carcinomas of the Colorectal Mucosa
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Lee Sang-Dae
Lee Tae-Jin Park Eon-Sub
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Abstract
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Background/Aims: Nuclear factor-¥êB p65 (NF-¥êB p65), nuclear factor-¥êB1 p50 (NF-¥êB p50) have been shown to play a role in cell proliferation, apoptosis, cytokine production, and oncogenesis. Recently, p38 mitogen- activated protein kinase (MAPK)/ NF-¥êB/ cyclin D1 signaling pathway has been shown to play an important part in the pathogenesis of human cancers. This study was designed to investigate the expression of NF-¥êB p65, NF-¥êB p50, p38 MAPK¥á, and cyclin D1 proteins in premalignant lesions of colon and colorectal adenocarcinoma.
Methods: Paraffin sections of 20 normal mucosa, 20 low-grade tubular adenoma, 20 high-grade tubular adenoma and 64 adenocarcinoma tissues were analysed immunohistochemically for the expression of NF-¥êB p65, NF-¥êB p50, p38 MAPK¥á, and cyclin D1 proteins.
Results: The expression of NF-¥êB p65, NF-¥êB p50, and p38 MAPK¥á proteins were significantly higher in adenocarcinoma tissue in comparison with that in normal mucosa, low-grade tubular adenoma, and high-grade tubular adenoma tissues. Expression of NF-¥êB p50 was more frequent in poorly differentiated histologic grade, presence of nodal metastasis, and advanced stage. Expression of p38 MAPK¥á protein was higher in advanced tumor stage, presence of nodal metastasis and advanced stage. Synchronous expression of NF-¥êB p65, NF-¥êB p50, p38 MAPK¥á, and cyclin D1 proteins were significantly higher in adenocarcinoma tissue.
Conclusions: With the increased expression of NF-¥êB p65, NF-¥êB p50, and p38 MAPK¥á proteins, p38 MAPK/ NF-¥êB/ cyclin D1 signaling pathway may play a role in the pathogenesis of colorectal carcinoma.
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KEYWORD
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Carcinoma, Colorectal, NF-¥êB p65, NF-¥êB p50, p38 MAPK¥á, Cyclin D1
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